When the novel was first serialised in Revue de Paris between 1 October and 15 December 1856, public prosecutors attacked it on the grounds that it was obscene. The resulting trial in January 1857 rendered it notorious. Following Flaubert's acquittal on 7 February 1857, Madame Bovary became a bestseller in April 1857 when it was published in two volumes. A seminal work of literary realism, the novel is now ranked among Flaubert's masterpieces, and one of the most influential literary works in history.

The novel deals with themes of betrayal, faith, family, marriage, Imperial Russian society, desire, and the differences between rural and urban life. The story centres on an extramarital affair between Anna and cavalry officer Count Alexei Kirillovich Vronsky that scandalises the social circles of Saint Petersburg and forces the young lovers to flee to Italy in pursuit of happiness, but after they return to Russia, their lives further unravel.